藓羽藻(Bryopsis hypnoides)核酮糖-1，5-二磷酸羧化酶／加氧酶的分离与活性测定     

田超  张炎  王广策  曾呈奎 《海洋与湖沼》2004,35(4):371-375

采用硫酸铵分部沉淀与凝胶过滤的方法，进行藓羽藻Rubisco的分离研究。结果表明，分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带，分别为Rubisco大亚基与小亚基；与菠菜相比，藓羽藻Rubisco大亚基分子量与菠菜基本相同，而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明，Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多，分离后活力有所上升，但仍比粗提液活力弱；在Rubisco活力测定过程中，藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同，在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。  相似文献   

注射生物胺对凡纳滨对虾免疫指标的影响   总被引：1，自引：0，他引：1  

胡发文  潘鲁青  杨慧赞 《热带海洋学报》2007,26(5):64-68

研究了注射生物胺(多巴胺、5-羟色胺)对凡纳滨对虾Litopenaeus vanna mei免疫指标的影响。结果表明,注射生物胺(10-9mol.尾-1)对凡纳滨对虾血细胞数量、血淋巴酚氧化酶活力、溶菌和抗菌活力影响显著(p<0.05),而对照组各免疫指标无明显变化;在实验时间内,注射生物胺处理组各免疫指标呈明显峰值变化,血细胞数量均在3h达到最小值,血淋巴酚氧化酶活力和溶菌、抗菌活力均分别于6h时达到最大值和最小值,且多巴胺处理组各免疫指标比5-羟色胺处理组变化更明显,其中大、小颗粒细胞和血淋巴酚氧化酶活力在12h后保持稳定,总血细胞和透明细胞数量18h后趋于稳定,血淋巴溶菌和抗菌活力在24h后稳定,而且稳定后各免疫指标与对照组均无显著差异。  相似文献   

溴素的有机化学──Ⅴ.溴代脂肪酸—2,3,5,6—四溴—1,4—苯二酯的合成     

崔伟  孙明昆  钱佐国  刘升一 《中国海洋大学学报(自然科学版)》1996,(3)

以1，4—苯二酚和不同的短链脂肪酸为基本原料，经溴代、加成、形成酰卤和酰化等反应，合成了六种新的溴化脂肪酸—2，3，5，6—四溴—1，4—苯二酯。通过对产物的碳、氢、溴等元素的定量分析，以及红外吸收光谱和氢核磁共振谱的研究，验证了化合物的结构。  相似文献   

基于LabView的松科2井项目管理系统          下载免费PDF全文

张恒春  闫家  谈晓丽  孟庆鸿  王稳石  曹龙龙  朱永宜  许洁 《探矿工程》2018,45(10):171-176

松科二井2013年正式启动,2018年5月完井,历时6年,最终井深7018 m。项目执行过程中,形成合同700多个、文件200多个、汇款流水1000多笔、报账流水1600多笔。针对松科二井合同、文件繁多,财务流程繁杂,涉及项目多的特点,基于LabVIEW平台设计了一套项目管理系统。该系统可全程记录松科二井上述合同、文件、汇款和报账信息,建立合同及对应批次汇款和报销的勾稽关系,具有模糊查询、修改、超链接等功能。  相似文献   

构造叠加晕结合五方位测井在甘肃省贾公台金矿寻找深部矿体中的应用          下载免费PDF全文

杨怀玉  蒙轸  刘杰  赵瑛  梁悦  贾新勇 《甘肃地质》2023,(1):20-28

贾公台金矿为甘肃党河南山地区规模最大的金矿床。为探索深部矿化、寻找盲矿体,在贾公台矿段开展原生晕研究,建立原生叠加晕轴向分带,发现贾公台金矿具反向分带特征。说明该矿体深部存在多期次成矿作用。结合地井五方位测井,预测深部3 200 m标高以下存在盲矿体。深部钻探发现了厚度大、品位高的多层金矿体,新增金资源量12吨,取得了显著找矿成果。构造叠加晕结合地井五方位测井成功圈定贾公台金矿深部矿体的实践,对同类矿床的深部找矿具有重要指导意义。  相似文献   

基于CMIP6模式的黄河上游地区未来气温模拟预估     

李纯  姜彤  王艳君  缪丽娟  李溯源  陈梓延  吕嫣冉 《冰川冻土》2022,44(1):171-178

利用第六次国际耦合模式比较计划（CMIP6）提供的5个气候模式,并结合基于地面气象站的CN05.1气象资料,评估了CMIP6模式对黄河上游地区1961—2014年气温变化的模拟能力。基于7个共享社会经济路径及代表性浓度路径（SSP-RCP）组合情景,结合多模式集合平均预估了2015—2100年黄河上游地区年均气温和季平均气温的时空变化规律。结果表明：多模式集合平均能较好地模拟黄河上游地区历史平均气温的空间分布格局与年变化。7个未来情景一致表明,2015—2100年黄河上游地区年平均气温呈现波动上升趋势［0.03～0.82 ℃?（10a）^-1］。其中,低辐射强迫情景下（SSP1-1.9、SSP1-2.6及SSP4-3.4）气温先呈现增加趋势,21世纪中期到达增幅峰值,之后增温呈现放缓趋势;而中、高辐射强迫情景下（SSP2-4.5、SSP3-7.0、SSP4-6.0及SSP5-8.5）气温表现为持续上升态势。空间上,未来气温增幅显著的区域位于黄河上游西部地区;时间上,呈现夏季增温快,春季增温慢。四季增温的空间分布呈现出一致特征,表现为西部增温强于东部,北部增温强于南部。研究结果可为黄河流域水资源管理及气候变化的适应性研究提供科学依据。  相似文献   

Rapid screening of Zr-containing particles from Chang’e-5 lunar soil samples for isotope geochronology: Technical roadmap for future study     

《地学前缘(英文版)》2022,13(3):101367

New samples returned by China Chang’e-5 (CE-5) mission offer an opportunity for studying the lunar geologic longevity, space weathering, and regolith evolution. The age determination of the CE-5 samples was among the first scientific questions to be answered. However, the precious samples, most in the micrometer size range, challenge many traditional analyses on large single crystals of zircon developed for massive bulk samples. Here, we developed a non-destructive rapid screening of individual zirconium-containing particle for isotope geochronology based on a Micro X-ray fluorescence analysis (µXRF). The selected particles were verified via scanning electron microscopy (SEM), 3D X-ray microscopy (XRM), and focused ion beam scanning electron microscopy (FIB-SEM) techniques, which showed that zirconium-bearing minerals with several microns were precisely positioned and readily suitable for site-specific isotopic dating by second ion mass spectrometry (SIMS). Such protocol could be also applicable in non-destructively screening other types of particles for different scientific purposes. We therefore proposed a correlative workflow for comprehensively studying the CE-5 lunar samples from single particles on nanometer to atomic scales. Linking various microscopic and spectromicroscopic instruments together, this workflow consists of six steps: (1) single-particle selection with non-destructive µXRF technique, (2) 2D/3D morphological and structural characterization with a correlative submicron 3D XRM and nanoscale resolution FIB-SEM imaging methods, (3) SEM analysis of the surface morphology and chemistry of the selected particle, (4) a series of microscopic and microbeam analyses (e.g., SEM, electron probe microanalysis, and SIMS) on the cross-section of the selected particle to obtain structural, mineralogical, chemical, and isotopic features from the micron to nanometer scale, (5) advanced 2D/3D characterization and site-specific sample preparation of thin foil/tip specimens on a microregion of interest in the selected particle with FIB-SEM technique, and (6) comprehensive analyses on the FIB-milled specimens at nanometer to atomic scale with synchrotron-based scanning transmission X-ray microscopy, analytic transmission electron microscopy, and atom probe tomography. Following this technical roadmap, one can integrate multiple modalities into a uniform frame of multimodal and multiscale correlated datasets to acquire high-throughput information on the limited or precious terrestrial and extraterrestrial samples.  相似文献   

Surface air temperature changes over the Tibetan Plateau: Historical evaluation and future projection based on CMIP6 models     

《地学前缘(英文版)》2022,13(6):101452

With its amplification simultaneously emerging in cryospheric regions, especially in the Tibetan Plateau, global warming is undoubtedly occurring. In this study, we utilized 28 global climate models to assess model performance regarding surface air temperature over the Tibetan Plateau from 1961 to 2014, reported spatiotemporal variability in surface air temperature in the future under four scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5), and further quantified the timing of warming levels (1.5, 2, and 3 °C) in the region. The results show that the multimodel ensemble means depicted the spatiotemporal patterns of surface air temperature for the past decades well, although with differences across individual models. The projected surface air temperature, by 2099, would warm by 1.9, 3.2, 5.2, and 6.3 °C relative to the reference period (1981–2010), with increasing rates of 0.11, 0.31, 0.53, and 0.70 °C/decade under the SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5 scenarios for the period 2015–2099, respectively. Compared with the preindustrial periods (1850–1900), the mean annual surface air temperature over the Tibetan Plateau has hit the 1.5 °C threshold and will break 2 °C in the next decade, but there is still a chance to limit the temperature below 3 °C in this century. Our study provides a new understanding of climate warming in high mountain areas and implies the urgent need to achieve carbon neutrality.  相似文献   

西园隧洞引水工程对滇池COD_Mn的影响分析     

李锦秀  刘树坤  李术军 《湖泊科学》1998,10(2):78-84

本文在滇池湖流特性研究的基础上,建立了滇池平面二维ＣＯＤＭｎ输移扩散数学模型, 模型对污染物进入滇池水体后的输移扩散规律进行模拟分析,对滇池周围西园隧洞引水工程第一系列污染控制工程措施对滇池主要污染指标ＣＯＤＭｎ的影响进行评价,为综合治理滇池提供了理论依据。  相似文献   

三峡工程蓄水运用以来水库排沙效果   总被引：1，自引：0，他引：1       下载免费PDF全文

陈桂亚  袁晶  许全喜 《水科学进展》2012,23(3):355-362

针对三峡水库蓄水运用以来排沙比问题,在深入研究三峡水库不同蓄水运用阶段水库排沙效果的基础上,着重研究各年年内蓄水排沙过程,系统地分析水库排沙效果的影响因素。结果表明:库区河道特性、入库水沙条件以及坝前水位的高低是水库排沙比变化的主要影响因素。2003年6月~2010年12月,水库排沙比为26.1%。水库排沙主要集中在汛期5~10月,排沙比为29.0%。尤其是在洪峰期间,库区水流流速较大,水流挟沙能力强,进入水库的泥沙大部分能输移到坝前,水库排沙比较大,当入库流量大于30000m³/s时,水库最大排沙比可达81.0%。此外,随着汛期坝前水位的抬高,水库排沙效果有所减弱,尤其是粗颗粒泥沙的多少也很大程度上影响水库排沙效果,水流的挟沙能力随着水流流速的减小而减小,粗颗粒泥沙的排沙比随之发生较全沙更为明显的减小。  相似文献